In the present Master Program we aim at providing you with advanced training in the area of Biotechnology, with application to the Marine Resources, seeking to apply current knowledge of modern marine biology and biotechnology to the study, protection and development of marine resources.
P1 Introduction to separation and recovery processes of marine biomolecules. P2 Selection criteria of separation and recovery processes. P3 Cell disruption methods, filtration, centrifugation. P4 Membrane and chromatographic processes. P5 Extraction, precipitation, crystallization and drying methods. P6 Process integration and scale-up.
Bioreactors a. Background: importance of bioreactors in marine biotechnology b. Reviews: microbial growth kinetics, product formation and substrate consumption and mass balances c. Heat transfer and sterilization d. Measurement and control of the main variables of a bioprocess e. Geometries and operation modes of bioreactors f. Bioreactors with non-mechanical agitation g. Scale-Up h. Scale-down i. Innovation in bioreactors and case studies: marine applications.
Biocatalysis a. Biocatalysis and biocatalysts – general properties and application examples. b. Enzyme kinetics – mechanisms, temperature and pH effect, inhibition. c. Kinetics in multiphase systems. d. Enzymes and cellular biocatalysts. e. Immobilization of biocatalysts. f. Biocatalysis in non-conventional media. g. Innovation in biocatalysis and case studies – marine biocatalysts
P1 – Microbiological diversity in the marine environment: Bacteria, Archaea and Eukarya (the particular case of marine fungi). Biotechnological potential of marine microorganisms. P2 – Microbiological laboratory techniques: 2.1 – Isolation, identification, growth and study of marine diversity. 2.2 – Metabolic and physiological laboratory study of marine microorganisms. 2.3 – Methodology for characterization and identification of marine microorganisms. 2.4 – Use of complementary biochemical and molecular tools frequently used in the characterization and identification of marine microorganisms (amplification of specific genes, 16S rRNA, …) 2.5 – Analyse experimental results; perform calculations needed for the interpretation of results; readjust and complementation of the experimental component, according to the results obtained.
Biology and diversity of photosynthetic organisms and the main groups of marine fauna
Methodologies for marine bioprospection
Compounds and molecules of marine origin with interest in marine biotechnology 4.1 Anti-oxidants 4.2 Anti-tumoral agents 4.3 Antifungal and antibacterial agents 4.4 Anti-inflammatory compounds 4.5 Enzymes 4.6 Other compounds and molecules of diverse interest
Entrepreneurship: From idea to business; Preliminary feasibility study; Financing and available options; Technology-based entrepreneurship.
Biotechnology on a global scale: the Biotechnology Cluster; the Marine Resources Cluster; the importance of intellectual property in biotechnology; legislation and constitution of biotech-based companies; obtaining financing.
Strategic thinking in bioentrepreneurship: Perception of opportunity; Aggregation of intellectual capital; Human and technological capital; Economic-financial capital.
Leveraging business opportunities: Synergies and strategic alliances; Spinoffs and the importance of connecting the academic and business environment; Legal aspects and support for the biotechnology and marine resources industry.
Analysis of investment projects in the area of Biotechnology 5.1 CANVAS Business Model
Communication of results 4.1. communication in science 4.2. Search for scientific content Scientific article writing 4.4. 4.4. Panel communication 4.5. Oral communication 4.6. Communicating with society
Research, Development and Innovation Projects 5.1 Project writing 5.2 Introduction to the management of a project
a) Students must choose 4 of the following curricular units
A. Pollutants, contaminants and environmental risks in the marine environment.
Types; Sources; Concentrations; Environmental impact and monitoring.
Legislative framework of marine environmental problems and their resolution. B. Integration of biotechnological steps in the reduction of environmental problems in the marine environment. C. Integration of biotechnological steps in the treatment of environmental problems in the marine environment. D. Exploitation of the potential of marine organisms in bioremediation processes E. Valorisation of by-products of marine origin.
Biotechnology in Aquaculture
The importance of biotechnology in aquaculture and its impacts.
The genetic variability in aquaculture and the physical bases of heredity. Quantitative genetics and principles of fishimprovement. Genetic markers for identification of phenotypic characteristics in aquaculture. Quantification of geneticvariability and statistical distributions. Heritability, types of selection and types of crossing (experimental design).
Molecular tools for diagnosis of viral and bacterial diseases.
Applications of genetic engineering in aquaculture: DNA construction; methods of gene transfer in fish; detection ofintegration and transgenic expression; results of genetic engineering technology in fish, regulation of GMOs; ethicalaspects.
Polyploidy in aquatic species.
DNA vaccines and their use in aquaculture.
Use of probiotics in aquaculture.
Biotechnological potential of algae aquaculture.
P1. Fundamental concepts and technologies in the field of Pharmaceutical Biotechnology: Introduction to pharmaceuticals, biopharmaceuticals and biotechnology. Pharmacokinetics and pharmacodynamics of biotechnological drugs. Future perspectives, economic considerations. P2. Development of industrial production processes of drugs: Discovery of biopharmaceuticals and their characterization; industrial production; analysis of the final product; preclinical, clinical and toxicological studies; regulatory agencies and patenting. P3. Fundamental lines of research in Clinical and Pharmaceutical Biotechnology: Interferon; Interleukins and tumor necrosis factors; Growth factors; Therapeutic hormones; Recombinant blood products and therapeutic enzymes; Antibodies, vaccines and adjuvants; Therapies based on nucleic acids and cells.
Food technology and processing fundamentals 1.1 – Role of functional properties of food components within sensory quality 1.2 – Extraction and texturization processes
Innovation and current trends in food production 2.1 – Nutraceuticals 2.2 – Edible films
Sources of marine derived food ingredients 3.1 – Algae 3.2 – Extremophiles 3.3 – Sponges 3.4 – Fish and seafood by-products
Animal cell and tissue culture: animal cell biology: metabolism; adhesion; proliferation; differentiation. Primary culture, subcultures, and cell lines. Equipment and culture media. Cryopreservation of animal cells. Experimental methods: viability, cytotoxicity, cell proliferation, types of cell death (apoptosis, necrosis, necroptosis, autophagy). Tissue engineering. Stem cells. Multidrug-resistant cells: model used to test new drugs. Coculture and 3D models. Ethical aspects. Plant cell and tissue technology: application in agricultural production, forestry, and biotechnology industries. Main systems and types of plant cell cultures, culture conditions and physiological effects. Importance and molecular mechanism of phytohormones. Somaclonal and epigenetic variation. Culture of suspension cells, embryogenesis, and protoplasts. Production of secondary metabolites. Genetic transformation of plant cells
Biomaterials and Biosensors
Biomaterials and biological materials, (BM): Definitions and classifications. Polymers and composites.
Biomaterials of marine origin, (BOM): Chitin and chitosan, Collagen, Alginates, Carrageenan, Biosilica. Other polymers of marine origin.
Types of biosensors (BS)
Materials and detection techniques, examples of biosensors (MB)
Applications to health, environment and marine biological processes (AB)
Holders of an academic bachelor’s degree following a 1st cycle of studies organized according to the principles of the Bologna process in the area of Marine Biology and Biotechnology, and related areas;
Holders of a bachelor’s degree that is recognized as meeting the objectives of a degree by the Scientific-Technical Council of ESTM in the area of marine biology and biotechnology or related areas;
Holders of an academic, scientific or professional curricula who is recognized as attesting the capacity to carry out this cycle of studies by the Scientific-Technical Council of ESTM.
English Language Proficiency.
State: Accredited Nº of years of Accreditation: 4 Date of Publication: 31/07/2022 A3ES Accreditation (updating)